Aim: The study was carried out with an idea to ascertain involvement of 
trace minerals in failure of conception in Knakrej cow. 
              
              Materials and 
Methods: The animals under study were comprised of 10 normal (Group-I) and 
20 repeat breeder (Group-II) Kankrej cows. Blood and estrual mucus of each 
repeat breeding and normally cycling cows were collected aseptically before 
breeding. The cervical mucus was diluted with triple glass distilled water at 
the rate of 1: 3 using vertex machine. Serum and diluted mucus was used for the 
trace minerals estimation. Results: The values observed for copper, iron, 
zinc and manganese in blood serum of normal and repeat breeding animals was 2.27 
± 0.05 vs 0.87 ± 0.02, 1.41 ± 0.02 vs 0.65 ± 0.01, 1.94 ± 0.01 vs 1.78 ± 0.02 
and 0.43 ± 0.02 vs 0.18 ± 0.01 ppm, respectively and in estrual mucus it was 
1.37 ± 0.10 vs 0.44 ± 0.06, 0.74 ± 0.07 vs 0.33 ± 0.05, 1.47 ± 0.10 vs 0.82 ± 
0.06 and 0.29 ± 0.04 vs 0.23 ± 0.03 ppm, respectively. All the values in blood 
serum and estrual mucus were significantly (p<0.01) higher in normal than repeat 
breeder cows except manganese in estrual mucus, which was non significantly 
higher in normal as compared to repeat breeder Kankrej cows. 
              
              Conclusion: 
All trace minerals was higher in blood serum of Group I animals as compared to 
Group II. In estrual mucus except manganese, all trace minerals was higher in 
normal cows than repeat breeder. Keywords: blood serum, estrual Mucus, 
repeat breeder, trace minerals